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2 edition of Studies of the deoxyribonucleotide kinases of strain L mouse fibroblasts. found in the catalog.

Studies of the deoxyribonucleotide kinases of strain L mouse fibroblasts.

Thomas John Griffith

Studies of the deoxyribonucleotide kinases of strain L mouse fibroblasts.

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  • 18 Currently reading

Published in [Toronto] .
Written in English

    Subjects:
  • Enzymes

  • Edition Notes

    ContributionsToronto, Ont. University.
    The Physical Object
    Paginationvii, 69 leaves.
    Number of Pages69
    ID Numbers
    Open LibraryOL20794584M

    In biochemistry, a ribonucleotide is a nucleotide containing ribose as its pentose component. It is considered a molecular precursor of nucleic tides are the basic building blocks of DNA and monomer itself from ribonucleotides forms the basic building blocks for RNA. However, the reduction of ribonucleotide, by enzyme ribonucleotide reductase (RNR), forms. In the present studies, mechanisms mediating skin toxicity were examined using a mouse skin construct model and a full-thickness human skin equivalent (EpiDerm-FT{sup TM}). In both systems, administration of the model sulfur mustard vesicant, 2-chloroethyl ethyl sulfide (CEES, {mu}M) at the air surface induced mRNA and protein. Protein kinase C epsilon type (PKCε) is an enzyme that in humans is encoded by the PRKCE gene. PKCε is an isoform of the large PKC family of protein kinases that play many roles in different tissues. In cardiac muscle cells, PKCε regulates muscle contraction through its actions at sarcomeric proteins, and PKCε modulates cardiac cell metabolism through its actions at Aliases: PRKCE, PKCE, nPKC-epsilon, protein . Body weight was recorded biweekly and metabolic parameters were measured. We also used mouse embryonic fibroblasts deficient in AMPK to study the role of AMPK in resveratrol-mediated effects in vitro. Results: Resveratrol increased the metabolic rate and reduced fat mass in wild-type mice but not in AMPKalpha1(-/-) mice. In the absence of.


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Studies of the deoxyribonucleotide kinases of strain L mouse fibroblasts. by Thomas John Griffith Download PDF EPUB FB2

Positron emission tomography (PET) is a molecular imaging modality with applications in cancer and other diseases. PET studies of immune function have been limited by a lack of specialized probes. The present study was conducted in order to clarify the patho-mechanism of mtDNA depletion in dGK deficiency by studying the mitochondrial and cytosolic dNTP pools in cycling and quiescent fibroblasts from a dGK deficient by: Unlabelled deoxynucleotides and [14C]AMP and [14C]GMP were commercial preparations.

Mouse fibroblast cells (strain L6oTM, a subline of Earle's L strain) were cultivated in medium CMRL Io66 (PARKER9) lacking thymidine and coenzymes.

The medium was supplemented (IO by vol.) with horse by: 6. Stromal fibroblast-specific deletion of mouse orthologs of several candidates resulted in the hyper-proliferation of mammary gland epithelium. Furthermore, a gene signature of human orthologs was selectively enriched in the tumor stroma of breast cancer patients, and depletion of these factors from normal human breast fibroblasts increased Cited by: 9.

Earlier work has shown that azidocytidine inhibits the growth and DNA synthesis of 3T6 mouse fibroblasts by inactivation of the enzyme ribonucleotide reductase.

The Erks, or MAP kinases, are activated in response to a dizzying array of extracellular ligands that act through a variety of cell surface receptor tyrosine kinases (RTK). The Drosophila melanogaster (D. melanogaster) ERK-A gene is a closely related member of the MAP kinase.

Ein wichtiges Modell für die Untergsuchung des molekularen Mechanismus der Vermehrung DNS-haltiger tierpathogener Viren ist Bessman, M. J.: Deoxyribonucleotide kinases in normal and virus and J. Melnick, and L. Piekarski: Enhanced thymidine phosphorylating activity of mouse fibroblasts (strain L-M)following vaccinia infection Cited by: 1.

The synthesis of deoxyribonucleotide is regulated by ATP, which functions as an allosteric activator; dATP, dGTP, dTTP, and dCTP depress their own synthesis via negative feedback regulation. View chapter Purchase book. Abstract. A recent analysis by Kirkland et al.

[Kirkland, D., Aardema, M., Henderson, L. and Müller, L. () Evaluation of the ability of a battery of 3 in vitro genotoxicity tests to discriminate rodent carcinogens and non-carcinogens. Sensitivity, specificity and relative predictivity.

Mutat. Res. 1–] demonstrated an extremely high false positive Cited by: Modeling and Experimental Analyses Reveals Signaling Plasticity in a Bi-Modular Assembly of CD40 Receptor Activated Available via license: CC.

Initial studies characterized fibroblast subsets obtained from the mouse lung 9, 19 and demonstrated that they possessed identifying morphological characteristics. Thy-1 + fibroblasts are generally spindle-shaped and synthesize large amounts of type I and III collagen.

Schematic illustration of the mouse p53 promoter showing the PF1 (TGA C TCT, black circle)-binding site. 20 (l) and mouse cyclin D1 promoter (m) showing the TRE element (TGT C TCA, red circle).

33 Cited by: As a harbinger of things to come, it was observed that mouse embryo fibroblasts (MEFs) lacking p27 Kip1 and p21 Cip1 did not stably express D-type cyclins and had >90% reduction in net cyclin D-dependent kinase activity, but nevertheless proliferated normally (Cheng et al.

This was reminiscent of the relationship between the G1 cyclins. Saul Kit's research works with 6, citations and reads, including: Strategy of Simian Virus   Neisseria meningitidis, the causative agent of meningitis and septicemia, is able to attach to and invade a variety of cell types.

In a previous study we showed that entry of N. meningitidis into human brain microvascular endothelial cells (HBMEC) is mediated by fibronectin bound to the outer membrane protein Opc, which forms a molecular bridge to by: studies were based on the study of cultured fibroblasts.

Regarding in vivo research, the bleomycin-induced experi- mental sclerotic mouse is a good model for studying the. Cellular senescence is the dynamic process of durable cell-cycle arrest.

Senescent cells remain metabolically active and often acquire a distinctive bioactive secretory phenotype. Much of our molecular understanding in senescent cell biology comes from studies using mammalian cell lines exposed to stress or extended culture periods.

While less well understood. Start studying BIO - Chapter 6. Learn vocabulary, terms, and more with flashcards, games, and other study tools.

Senescence is observed in both human and mouse cells, however, there are fundamental differences in how senescence is controlled between the two species.

Human fibroblasts undergo replicative senescence as a result of telomere shortening. In contrast, mouse fibroblasts do not senesce when grown at a physiological oxygen by: 1.

The c-Jun N-terminal kinases (JNKs), with its members JNK1, JNK2, and JNK3, is a subfamily of (MAPK) mitogen-activated protein kinases.

JNK signaling regulates a wide range of cellular processes, including cell proliferation, differentiation, survival, apoptosis, and inflammation. Dysregulation of JNK pathway is associated with a wide range of immune disorders and.

Deficient enzymatic activity of the mitochondrial deoxyribonucleoside kinases deoxyguanosine kinase (DGUOK) or thymidine kinase 2 (TK2) cause mitochondrial DNA (mtDNA)-depletion syndromes in humans. Here we report the generation of a Tk2-deficient mouse strain and show that the mice develop essentially normally for the first week but from Cited by: Equal amounts of nucleotides are made 2.

To synthesize either of these molecules you need energy input. When making AMP, you use energy in the form of GTP, and when making GMP you use energy in the form of ATP 3.

The end synthesis is the Purine nucleotide cycle 4. GTP and ATP can participate in RNA synthesis directly. Studies on the metabolism of 5-iodo-2'-deoxycytidine in vitro.

Purification of nucleoside deaminase from mouse kidney. J Biol Chem. May; –Cited by: [Show full abstract] very old normal human diploid fibroblasts, as well as in a variant human fibroblast strain with an altered replicative life span.

These studies indicated that there are. Here, we study deoxynucleotide metabolism in human fibroblasts from a patient with a homozygous missense mutation substituting a glycine by a valine residue in the diferric iron center of p53R2, thereby impeding the generation of the tyrosyl radical.

The patient died in early infancy with severe depletion of mtDNA in muscle. We obtained quiescent fibroblasts by Cited by: The cardiac fibroblast is a remarkably versatile cell type that coordinates inflammatory, fibrotic and hypertrophic responses in the heart through a complex array of intracellular and intercellular signaling mechanisms.

One important signaling node that has been identified involves p38 MAPK; a family of kinases activated in response to stress and inflammatory stimuli that modulates Cited by: 2. Mitochondrial DNA (mtDNA) depletion is associated with heterogeneous clinical phenotypes.

The recent identification of the mutated genes in three groups of patients with mtDNA depletion had Cited by: Studies on the telomerase-deficient mouse have revealed that although the loss of telomerase activity per se and no discernible impact on the response to ionizing radiation, however late generation telomerase deficient (Terc/) mice with dysfunctional telomeres imparted radiosensitivity syndrome associated with enhanced mortality.

Interestingly, the gastrointestinal crypt Author: Tej K. Pandita. Recent studies propose that depletion of deoxyribonucleotide (dNTP) pools is a main cause of replication stress during OIS. Specifically, Aird et al. reported that the ribonucleotide reductase (RR) subunit M2 (RRM2), the rate-limiting enzyme in dNTP synthesis, is downregulated prior to growth arrest in oncogenic Ras-expressing by: 4.

The protein is not cell cycle-regulated and can provide deoxynucleotides to quiescent mouse fibroblasts. Here we investigate the in situ activities of the R1-p53R2 complex and two other enzymes of the de novo pathway, dCMP deaminase and thymidylate synthase, in confluent quiescent serum-starved human fibroblasts in experiments with [5- 3 H.

The three major DNA replication fidelity determinants are nucleotide selectivity, proofreading, and mismatch repair. Defects in the two latter determinants are now firmly associated with cancer.

Nucleotide selectivity is affected by changes in the absolute or relative concentrations of dNTPs. Here, we show that hemizygous SAMHD1 +/− mouse embryos Cited by: 26 January The Erratum to this article has been published in Genome Biology Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2.

Authors: Michael I Love, Wolfgang Huber and Simon Anders. Content type: METHOD. 5 December Differential expression analysis for sequence count data. In enzymology, a deoxynucleoside kinase (EC ) is an enzyme that catalyzes the chemical reaction.

ATP + 2'-deoxynucleoside ⇌ ADP + 2'-deoxynucleoside 5'-phosphate. Thus, the two substrates of this enzyme are ATP and 2'-deoxynucleoside, whereas its two products are ADP and 2'-deoxynucleoside 5'-phosphate. This enzyme belongs to the family of BRENDA: BRENDA entry.

Grassilli E, Bellesia D, Salomoni P, Croce MA, Sikora E, Radiszewska E, Tesco G, Vergelli M, Latorraca S, Barbieri D, Fagiolo U, Santacaterina S, Amaducci L, Sorbi S, Franceschi C () C-fos/c-jun expression and AP-1 activation in skin fibroblasts from centenarians.

Biochem Biopys Res Commun – CrossRef Google ScholarCited by: 2. Hassani SN, Totonchi M, Farrokhi A, Taei A, Larijani MR, Gourabi H, Baharvand H () Simultaneous suppression of TGF-beta and ERK signaling contributes to the highly efficient and reproducible generation of mouse embryonic stem cells from previously considered refractory and non-permissive by: 6.

We developed a telomerase-positive, infinite life span human fibroblast cell strain (MSU) by transfection of a v-MYC oncogene and spontaneous over-expression of transcription factors SP1/SP3.

Loss of expression of p14 ALT and enhanced expression of SPRY2 gave rise Cited by: 6. BiochemistryA Comparison of Methanobactins from Methylosinus trichosporium OB3b and Methylocystis Strain SB2 Predicts Methanobactins Are Synthesized from Diverse Peptide Precursors Modified To Create a Common p67/MetAP2 Suppresses K-RasVMediated Transformation of NIH3T3 Mouse Fibroblasts in Culture and in Athymic Mice.

Avijit. Alan Hall showed the specificity of Rho in the stimulation of focal adhesions and stress fibres formation in fibroblasts in the presence of extracellular factors in He first realised that the addition of bovine fetal calf serum (FCS) to Swiss 3T3 cells increased the polymerisation of actin and assembly of stress fibres.

Cerebral cortical neurons have a high vulnerability to the harmful effects of hypoxia. However, the brain has the ability to detect and accommodate to hypoxic conditions. This phenomenon, known as preconditioning, is a natural adaptive process highly preserved among species whereby exposure to sub-lethal hypoxia promotes the acquisition of tolerance to a Cited by: To elucidate the participation of this enzyme further, in this study the authors examined several parameters related to pol II during the cycle of vaccinia virus infection in L-strain fibroblasts, HeLa cells, and L/sub 6/H/sub 9/ rat myoblasts.

@article{osti_, title = {Sodium arsenite induces chromosome endoreduplication and inhibits protein phosphatase activity in human fibroblasts}, author = {Huang, Rong-Nan and Ho, I-Ching and Yih, Ling-Hui}, abstractNote = {Arsenic, strongly associated with increased risks of human cancers, is a potent clastogen in a variety of mammalian cell systems.Function.

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized 1 and the related AKT2 are activated by platelet-derived growth activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through Aliases: AKT1, AKT, CWS6, PKB, PKB-ALPHA.

Introduction. Mammalian cells can obtain deoxyribonucleoside triphosphates (dNTPs) via two pathways—de novo synthesis and salvage (Fig.

1A) ().A key player in de novo dNTP synthesis is ribonucleotide reductase (RNR), 3 which converts NDPs to dNDPs that are subsequently phosphorylated to dNTPs by nucleoside diphosphate kinases ().Mouse RNR Cited by: 3.